Year: 2016 | Month: April | Volume 9 | Issue 2
Enhanced antioxidant system reprogrammes oxidative stress through hypersensitive response in leaves of rice plant challenged with Aspergillus species
Abstract:
Nitric Oxide (NO) is involved in diverse physiological processes in plants. As a developmental regulator, it promotes germination, leaf extension and root growth, and also delays leaf senescence and fruit maturation. As modulator of disease resistance, it triggers hypersensitive cell death or Hypersensitivity Response (HR) and activates the expression of several defense genes. This wide variety of effects reflects the basic signaling mechanisms that are utilized by virtually all living organisms. The ubiquity of NO reactions as well as the finding that the biochemical and molecular mechanisms underlying many physiological processes are well conserved between diverse species has opened the exploration of NO chemistry in different organisms. Experiments were conducted in Department of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University to study the NO mediated HR during interaction of Aspergillus sp. with rice plants. The pathogen (Aspergillus sp) was infiltrated in leaves of rice plants (Oryza sativa), and the responses were observed after 6, 12, 24 and 48 h of infection. The experiment was laid out in Factorial Complete Randomized Design which consisted of 8 treatments, 3 replications. Different biochemical parameters like Nitrite content, Proline, H2O2, SOD, Ascorbate Peroxidase (APX), Total Phenol Content (TPC), Phenyl ammonia lyase (PAL) were measured. PAL and TPC measures the quantity of Systemic Acquired Resistance (SAR) developed in plants against pathogen attack. Among the above mentioned parameters except nitrite, proline and H2O2, all other parameters showed maximum activity at 48 h treatment. The results indicate that HR was developed during rice-Aspergillus interaction through enhanced anti-oxidant system, possibly mediated through NO, thereby restricting pathogen’s spread after 12 to 48 h of infection of rice leaves with Aspergillus sp.
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